Figure 3.

Secondhand smoke exposure (SHSe) alters whole blood transcriptional profiles in cystic fibrosis (CF). (A) Class comparisons using linear models adjusted for age and race (p<0.01; Benjamini-Hochberg corrected flase discovery rate (FDR) and ≥1.25-fold change), in children with CF and SHSe (n=18) vs age-matched healthy controls (n=12) and children with CF and non-SHSe (n=10) vs the same healthy controls, identified 2188 differentially expressed transcripts that were present in either of these conditions. Infants were not analysed. Transcripts are organised in a heatmap format where each row represents a single transcript and each column represents a subject sample. Red colour indicates overexpression and blue colour underexpression of a transcript compared with the median expression of healthy controls (yellow). (B) Two prostaglandin transcripts (PTGR2 and PTGES3) were found to be significantly underexpressed in children with CF exposed to SHSe as compared with those not exposed to SHSe. (C) Modular expression in children with CF exposed to SHSe (n=18) and not exposed to SHSe (n=10), each group was compared with matched healthy controls (n=12). Children with CF exposed to SHSe demonstrated significant overexpression of inflammation genes and greater suppression of protein synthesis and plasma cell-related transcripts. The intensity of the modules (dots) indicates the proportion of overexpressed (in red) or underexpressed (in blue) transcripts within each module. Numeric values indicate the exact percentage of transcripts expressed in each specific module. A blank dot indicates that <10% of the genes in the module were differentially expressed.