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. Author manuscript; available in PMC: 2020 Nov 5.
Published in final edited form as: Thorax. 2019 Jan 19;74(3):237–246. doi: 10.1136/thoraxjnl-2018-211845

Figure 4.

Figure 4

Secondhand smoke exposure (SHSe) alters cystic fibrosis (CF) macrophage prostaglandin D2(PGD2) production. (A) Cytotoxicity as %LDH release was measured in child and adult CF monocyte-derived macrophage supernatants in response to increasing concentrations of cigarette smoke extract (CSE), 24 hours challenge, n=3. (B) Changes in the arachidonic acid metabolite PGD2 were measured in CF macrophage supernatants in response to increasing concentrations of CSE during a 24 hours challenge. (C) PGD2 expression was determined by western blot analysis in CF and non-CF macrophages in response to exposure to a 0% or 15% CSE, n=3, representative image shown.