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. 2020 Oct 22;10:552907. doi: 10.3389/fonc.2020.552907

Figure 2.

Figure 2

Regulatory effect of SNHG1 on proliferation of PCa cells SNHG1 by targeting EZH2. (A) FISH showed the subcellular location of SNHG1 in LNCaP and PC3 cells (red), and the nuclei were stained with DAPI (blue). (B, C) RNA Pull-down assay forward detected the binding of SNHG1 and EZH2 in LNCaP and PC3 cells; (D, E) RNA IP reverse verification of the binding of SNHG1 and EZH2 in LNCaP and PC3 cells; (F, G) MTT assay interfered with SNHG1, EZH2 down-regulated or interfered with SNHG1 while overexpressing EZH2, affecting the proliferation in LNCaP and PC3 cells; (H) Colony formation assay detected LNCaP and PC3 cells transfected with SNHG1 siRNA, EZH2 siRNA or SNHG1 siRNA+EZH2 after cell colony formation. *P < 0.05 and **P < 0.01 vs. NC group; ##P < 0.01 vs. si-SNHG1 + con group.