Figure 5.
Effect of SNHG1 binding EZH2 on autophagy in LNCap and PC-3 cells. (A–B) Immunofluorescence staining detected the formation of the LC3 spots with the interference of SNHG1 in LNCaP and PC3 cells and the down-regulation of EZH2 or the interference of SNHG1 and simultaneous overexpression of EZH2. (C) Western blot detected the expression level of autophagy-related proteins LC3-II, Beclin-1 and P62 protein in LNCaP and PC3 cells transfected with NC, si-SNHG1, si-EZH2, si-SNHG1+con, si-SNHG1+EZH2.