TABLE 2.

Efficiency of the hydrolysis of substrates Glp-Phe-Ala-pNA and Glp-Phe-Gln-pNA by cysteine peptidases of C1 family (n = 3, ±SE).

Enzyme	Substrate	kcat/KM, mM–1 min–1
Papain	Glp-Phe-Gln-pNA	1,410 ± 150
	Glp-Phe-Ala-pNA	640 ± 80
Ficin	Glp-Phe-Gln-pNA	490 ± 50
	Glp-Phe-Ala-pNA	180 ± 20
Cathepsin B human*	Glp-Phe-Gln-pNA	44 ± 2
	Glp-Phe-Ala-pNA	34 ± 2
Cathepsin L T. castaneum	Glp-Phe-Gln-pNA	190 ± 24
	Glp-Phe-Ala-pNA	33 ± 4
Cathepsin L human*	Glp-Phe-Gln-pNA	140 ± 27
Cathepsin L human**	Glp-Phe-Ala-pNA	33 ± 4

*Recombinant preparation. **From Semashko et al. (2014).