Fig. 2. Poly(A) and heparin induce LLPS and a reentrant phase transition with TDP-43 LCD.

a Turbidity of TDP-43 LCD as a function of poly(A) concentration (left) and representative bright field microscopy images of droplets in the presence of different concentrations of poly(A) (right), indicating that low concentrations of poly(A) promote condensation while high concentrations result in decondensation. b Turbidity of TDP-43 LCD as a function of heparin concentration (left) and representative bright field microscopy images of droplets in the presence of different concentrations of heparin (right). Experiments were performed using 5 μM TDP-43 LCD in a 20 mM potassium phosphate buffer, pH 6.0. Scale bars: 10 μm. (n ≥ 3 technical replicates). All data are presented as mean values ± standard deviation.