Table 1. Linear Regression (n = 5 × 3), Range, LOD and LOQ (n = 6), Precision (%, RSD) (n = 3), and Recovery (%) (n = 3) in the Developed UHPLC–PDA for Withanoside IV, Withanoside VII, Withanoside V, Withaferin A, 12-Deoxywithastramonolide, Withanolide A, Withanone, Withanolide B, Viscosalactone B, 27-Hydroxywithanone, and Dihydrowithaferin A.

analyte	retention time (min)	range (μg/mL)	linear regression equation	r2	LOD (μg/mL)	LOQ (μg/mL)	Mean average recovery (±SD)	interday precision (RSD %)	intraday precision (RSD %)	accuracy (RE)a
withanoside IV	15.32	1–150	y = 8529.4x + 13,360	0.999	0.35	1.061	100.11 ± 0.631	1.5	2.4	0.19
withanoside VII	16.85	1–150	y = 2882.1x – 6579.5	0.9983	0.362	1.098	94.61 ± 1.179	2.9	3.2	0.16
viscosalactone B	17.51	1–150	y = 2882.1x – 6579.5	0.9983	0.275	0.834	94.94 ± 3.246	3.5	3.8	0.15
27-hydroxywithanone	17.80	1–150	y = 24,932x + 3933	0.9989	0.248	0.755	94.85 ± 8.716	4.0	3.4	0.76
dihydrowithaferin A	20.12	1–100	y = 35,993x – 5549.6	0.9999	0.242	0.734	96.37 ± 2.709	2.6	2.5	0.36
withaferin A	21.34	1–150	y = 18,637x – 10,803	0.9989	0.257	0.778	93.71 ± 2.075	2.6	2.4	0.01
withanoside V	21.77	1–150	y = 9646.3x – 5395.6	0.9987	0.330	1.000	96.31 ± 3.423	3.6	1.5	0.28
12-deoxywithastramonolide	23.02	1–150	y = 25,926x – 9385.5	0.9998	0.213	0.646	94.08 ± 1.094	3.2	3.6	0.20
withanolide A	24.57	1–150	y = 27,113x + 19,186	0.9997	0.219	0.665	95.45 ± 3.071	2.6	1.9	0.19
withanone	24.84	1–150	y = 21,243x – 1606.4	0.9998	0.236	0.716	84.77 ± 0.547	3.1	1.2	0.22
withanolide B	30.18	1–150	y = 17,918x + 10,741	0.9997	0.223	0.675	99.07 ± 5.967	3.1	2.0	0.22

^aAccuracy (expressed in relative error) for the interday and intraday precisions