Table 2. UHPLC–PDA-Based ESI/MS–MS Identification and Fragmentation of Withanolides and Withanosides.

sr. no.	retention time (min)	analyte	molecular formula	molecular weight	polarity	precursor ion	MS/MS
1	15.67	withanoside IV	C40H62O15	782	–ve	781	[C40H61O15]- 781 – (m/z 764), (m/z 619), (m/z 221)
2	17.11	withanoside VII	C40H62O15	782	+ve	783	[C40H63O15]+ 783 – (m/z 441), (m/z 423), (m/z 405), (m/z 279)
3	18.12	viscosalactone B	C28H40O7	488	+ve	489	[C28H41O7]+ 489 – (m/z 453), (m/z 435), (m/z 317), (m/z 299), (m/z 281)
4	18.30	27-hydroxywithanone	C28H38O7	486	+ve	487	[C28H39O7]+ 487 – (m/z 429), (m/z 319), (m/z 297), (m/z 264)
5	20.70	dihydrowithaferin A	C28H40O6	472	+ve	473	[C28H41O6]+ 473 – (m/z 301), (m/z 283)
6	20.94	withaferin A	C28H38O6	470	–ve	469	[C28H37O6]- 469 – (m/z 451), (m/z 436), (m/z 311), (m/z 122)
7	22.45	withanoside V	C40H62O14	766	+ve	767	[C40H63O14]+ 767 – (m/z 425), (m/z 407), (m/z 253)
8	24.09	12-deoxywithastramonolide	C28H38O6	470	+ve	471	[C28H39O6]+ 471 – (m/z 171), (m/z 299)
9	25.80	withanolide A	C28H38O6	470	–ve	469	[C28H37O6]- 469 – (m/z 451), (m/z 433), (m/z 283)
10	26.10	withanone	C28H38O6	470	–ve	469	[C28H37O6]- 469 – (m/z 451), (m/z 433), (m/z 297)
11	30.65	withanolide B	C28H38O5	454	+ve	455	[C28H39O5]+ 455 – (m/z 437), (m/z 327), (m/z 289), (m/z 261)