Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Nov 4;11:5581. doi: 10.1038/s41467-020-19319-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — Left column: Cell-type-specific OCRs identified by ATAC-seq and nearby genes: (a) glutamatergic (BDNF), (b) GABAergic (DLX6), and (c) oligodendrocytes (CNDP1). The horizontal gray bars denote OCR assayed in directed transcription via transgenesis. Right: Representative immunofluorescent images showing mCherry (red) expression in 30 µm thick sagittal sections from (a) BDNF and (c) CNPD1 transgenic mice. In (a), specific mCherry expression is identified in Layer V of the cortex and in hippocampus. In (b) representative images of mCherry (red) staining in the cortex of DLX6 transgenic mice (top panel) and double labeling with NeuN (green; bottom panel), showing expression restricted to neurons and scattered in the cortex, similar to the distribution of GABAergic interneurons. In (c) mCherry expression is shown to be restricted to white matter. Four image frames of three independent brain slices per each mouse were analyzed (BDNF enhancer n = 5; DLX6 enhancer n = 6; CNDP1 enhancer n = 4).