Figure 4.

Proliferation of cells cultivated with different shell contents P@GNF for 1, 3, and 5 days (n = 3). NIH3T3 cells were mixed with P@GNF and seeded in a nontreated 48-well plate. WST-1 solution was added to each well and incubated for 1 h at 37 °C, under 5% CO₂ in an incubator in the dark. Supernatant was collected, and the absorbance at 450 nm was measured. (*) shows statistical significance (p < 0.05), which was evaluated by one-way ANOVA.