In
vitro cultivation of NIH3T3 cells with xP@GNF.
(a) Confocal laser scanning microscopy images of NIH3T3 cells with
100P@GNF, 80P@GNF, 50P@GNF, and 0P@GNF cultivated for 12 h and stained
with 4′,6-diamidino-2-phenylindole (DAPI) (blue, nucleus) and
Alexa Fluor 488 Phalloidin (green, F-actin) (scale bar = 50 μm).
(b) Elongation factor was calculated by the following equation: elongation
factor = (long axis)/(short axis). Long and short axes were measured
by ImageJ (n > 20). (*) shows statistical significance
(p < 0.05), which was evaluated by one-way ANOVA.