Fig. 4.

Tau mAbs inhibit AD-tau seeded aggregation of endogenous mouse tau in primary neurons. a Quantification of immunocytochemistry detection of AD-tau seeded insoluble mouse tau in primary neurons detected by the mouse-tau specific R2295M antibody. Statistical significance was determined relative to non-specific IgG control, using one-way ANOVA with Dunnett’s post-hoc analysis; *p < 0.05, **p < 0.01, n = 3–4 biological replicates each consisting of 3 technical replicate wells per plate. b Representative immunofluorescent staining images of insoluble mouse tau aggregates in primary neurons induced by AD-tau seeding detected by the mouse tau specific rabbit polyclonal antibody R2295M (green) and DAPI (blue). Tau5, DMR7, and SKT82 show inhibition of seeded tau pathology. c Confocal microscopy images of AD-tau induced aggregates of mouse tau in primary neurons detected by the mouse tau specific antibody T49 (green) and dendritic processes stained with MAP2 (red) with nuclei stained with DAPI (blue). Areas of MAP2 and mouse tau aggregate colocalization are indicated by white arrows. d Immunoprecipitation of tau from AD-tau extracts by IgG, SKT82, or DMR7. Bound and unbound fractions evaluated by western blot with total tau antibody (17025)