Fig. 2.

PD-MSCs^PRL-1 inhibit adipogenesis in OFs from GO patients. a Schematic diagram describing naïve PD-MSCs (Naïve (+)) and PD-MSCs^PRL-1 (PRL-1 (+)) coculture with normal (n = 3; individual open circle) and GO-derived OFs (GO-OF; n = 3; individual open circle) that underwent adipogenic differentiation. During the first 4 days, adipogenesis was induced in normal and GO-OFs. For 10 days, normal and GO-OFs were maintained and underwent adipogenic maturation. Naïve PD-MSCs and PD-MSCs^PRL-1 were cocultured in the transwell insert system for 24 h. b Representative images of adipogenic differentiation of normal and GO-OFs cocultured with naïve and PD-MSCs^PRL-1. Scale bars: 100 μm. c qRT-PCR analysis of mRNA expression of adipogenic markers in normal and GO-OFs that underwent adipogenic differentiation and were cocultured with naïve PD-MSCs and PD-MSCs^PRL-1. (mean ± SD *p < 0.05 compared with the noncoculture (−) groups) (mean ± SD #p < 0.05 compared with the naïve coculture groups; individual open circle). Statistic significances were determined by nonparametric Kruskal-Wallis test