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. 2020 Nov 5;11:469. doi: 10.1186/s13287-020-01982-3

Table 1.

Primer sequences using reverse transcription polymerase chain reaction

Genes Primer sequences Tm
Oct4 Forward 5′-AGTGAGAGGCAACCTGGAGA-3’ 52
Reverse 5′-GTGAAGTGAGGGCTCCCATA-3’
Nanog Forward 5′-TTCTTGACTGGGACCTTGTC-3’ 52
Reverse 5′-GCTTGCCTTGCTTTGAAGCA-3’
Sox2 Forward 5′-GGGCAGCGTGTACTTATCCT-3’ 52
Reverse 5′-AGAACCCCAAGATGCACAAC-3’
HLA-G Forward 5′-GCGGCTACTACAACCAGAGC-3’ 58
Reverse 5′-GCACATGGCACGTGTATCTC-3’
TERT Forward 5′-GAGCTGACGTGGAAGATGAG-3’ 55
Reverse 5′-CTTCAAGTGCTGTCTGATTCCAATG-3’
AFP Forward 5′-ATGCTGCAAACTGACCACGC-3’ 55
Reverse 5′-GCTTCGCTTTGCCAATGCTT-3’
Albumin Forward 5′-TGAGTTTGCAGAAGTTTCCA-3’ 60
Reverse 5′-CCTTTGCCTCAGCATAGTTT-3’
β-actin Forward 5′-TCCTTCTGCATCCTGTCAGCA-3’ 58
Reverse 5′-CAGGAGATGGCCACTGCCGCA-3’