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. 2020 Aug 28;2020:8831735. doi: 10.1155/2020/8831735

Figure 4.

Figure 4

ANP promotes neurite outgrowth of dissociated SGNs in vitro. Immunohistochemical analysis of dissociated SGNs maintained in culture medium alone (a), or treated with 20 ng/mL BDNF (b), 100 nM ANP (c), 1 μM ANP (d), 1 μM 8-pCPT-cGMP (e), or 1 μM ANP plus 1 μM KT5823 (f) for 5 d. Neurons were labeled with TuJ1 (green), while nuclei were stained with DAPI (blue). Scale bars = 100 μm. In each experimental culture, three SGNs seeded-culture dishes were studied for neurite outgrowth, and the cell number (g) and average neurite outgrowth length (h) of dissociated SGNs were calculated. Results are expressed as mean ± SEM (∗P < 0.05, versus negative control samples; #P < 0.05, versus positive control samples/BDNF; n.s., P > 0.05).