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GCAFs promote VM formation of GBC-SD cells in vitro (Phase contrast microscopy on 3-D cultures, × 200; A: Matrigel, B: rat-tail collagen-matrix). The tube number of vasculogenic-like networks in GBC-SD + GCAFs groups was significantly more than that of GBC-SD and GBC-SD + NFs groups (all *P < 0.05); furthermore, PAS positive, cherry-red materials found in granules and patches in the cytoplasm of GBC-SD cells appeared around the signal cell or cell clusters
