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. 2020 Nov 5;21:272. doi: 10.1186/s13059-020-02183-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Condensin depletion causes loss of chromatin integrity in interphase. a Typical fluorescent images of exponentially growing (cycling) and G2-arrested cells in the presence (cut14⁺) or absence (cut14^SO) of condensin. DNA, stained by DAPI, and mCherry-Atb2 (microtubules) are shown in green and magenta, respectively. Scale bar is 10 μm. b Distribution of cell lengths in cycling cells or following G2 arrest in cut14⁺ and cut14^SO strains (median cell lengths were as follows: cycling cells 9.7 μm, G2-arrested cut14⁺ cells 12.9 μm, and G2-arrested cut14^SO cells 14.3 μm). c Condensin depletion in G2-arrested cells was confirmed by western blotting. As, asynchronous cell culture; G2, cells arrested in G2 phase by 1NM-PP1. Cut14-AID was detected by α-AID antibody. α-TAT1 served as a loading control. d DNA volume quantified by DAPI staining. Boxplot of DNA volume in cut14⁺ control cells and following Cut14 depletion (cut14^SO). Data from three biological replicates, identified by their distinct shapes, is compiled in one boxplot. ***p = 7.6 × 10⁻⁸. Pseudocolor images of DAPI-stained DNA in cut14⁺ and cut14^SO are shown as reference. Scale bar is 1 μm. e Typical images of chromosomal distance, visualized by LacI-GFP at the lys1 locus (green, bottom left) and TetR-tdTomato at 1.95 Mb on chromosome I (red, bottom middle) in cut14⁺ and cut14^SO. DNA stained by DAPI is shown in blue (bottom right). f Chromosomal distances in cut14⁺ and cut14^SO cells are displayed as in d. **p = 0.0012