Fig. 2.
Biophysical evaluation of NF1 mediated hydrolysis on KRAS G13D. a Stimulation of the GTPase activity of KRAS 4B proteins by NF1–333. Reactions contained 1 μM KRAS 4B and 100 nM NF1–333. The arrow indicates the time point of NF1–333 addition. b The rates (kobs) of phosphate (Pi) release. The numbers above the bars indicate the fold stimulation for the NF1-catalyzed reaction (100 nM NF1–333) over the unstimulated reaction (0 nM NF1–333) for each KRAS 4B protein (1 μM). n = 2. c the rate of Pi release as a function of NF1–333 concentration for each KRAS 4B protein. n = 2