| Gene | Primers (5′–3′) |
|---|---|
| GAPDH (human) | Forward: GGAGCGAGATCCCTCCAAAAT |
| Reverse: GGCTGTTGTCATACTTCTCATGG | |
| Galectin-9 (human) | Forward: GGACGGACTTCAGATCACTGT |
| Reverse: CCATCTTCAAACCGAGGGTTG | |
| GAPDH (mice) | Forward: AGGTCGGTGTGAACGGATTTG |
| Reverse: TGTAGACCATGTAGTTGAGGTCA | |
| IFN-γ (mice) | Forward: ATGAACGCTACACACTGCATC |
| Reverse: CCATCCTTTTGCCAGTTCCTC | |
| IL-17 (mice) | Forward: TTTAACTCCCTTGGCGCAAAA |
| Reverse: CTTTCCCTCCGCATTGACAC | |
| EBI3 (IL-35, mice) | Forward: CTTACAGGCTCGGTGTGGC |
| Reverse: GTGACATTTAGCATGTAGGGCA | |
| T-bet (mice) | Forward: AGCAAGGACGGCGAATGTT |
| Reverse: GGGTGGACATATAAGCGGTTC | |
| RORyt (mice) | Forward: GACCCACACCTCACAAATTGA |
| Reverse: AGTAGGCCACATTACACTGCT | |
| Foxp3 (mice) | Forward: CCCATCCCCAGGAGTCTTG |
| Reverse: ACCATGACTAGGGGCACTGTA |
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