Table 8.
Primers Sequences and target genes used for quantitative real-time PCR
| Genesa | Gene full name | Primer sequence (5′-3′) | Accession No. |
|---|---|---|---|
| PGA5 | Pepsinogen A |
F-TCCGTCTACCTGAGCAAGGAT R- AAGCAGGCGACGTACTTGTT |
NM_204878.1 |
| PGC | Pepsinogen C |
F-ATCGGGATTGAGGA↓CTTCGC R- TGAAGACCTGGTTGGGAACG |
NM_204877.2 |
| AMY2A | Pancreatic alpha 2A amylase |
F-CGGAGTG↓GATGTTAACGACTGG R-ATGTTCGCAGACCCAGTCATTG |
NM_001001473.2 |
| PNLIP | Pancreatic lipase |
F-GCATCTGGGAAG↓GAACTAGGG R- TGAACCACAAGCATAGCCCA |
NM_001277382.1 |
| CCK | Cholecystokinin |
F-AGGTTCCACTGGGAGGTTCT R-CGCCTGCTGTTCTTTAGGAG |
XM_015281332.1 |
| CELA1 | Chymotrypsin-like elastase family, member 1 |
F-AGCGTAAGGAAATGGGGTGG R-GTGGAGACCCCATGCAAGTC |
XM_015300368.1 |
| GLUT1 | Glucose transporter-1 (SLC2A1) |
F-TCCTCCTGATCAACCGCAAT R-TGTGCCCCGGAGCTTCT |
NM_205209.1 |
| GLUT2 | Glucose transporter-2 (SLC2A2) |
F-TGATCGTGGCACTGATGGTT R-CCACCAGGAAGAC↓GGAGATA |
NM_207178.1 |
| CAT1 | Cationic amino acid transporter-1 (SLC7A1) |
F-CAAGAGGAAAACTCCAGTAATTGCA R- AAGTCGAAGAGGAAGGCCATAA |
XM_015277945.1 |
| CAT2 | Cationic amino acid transporter-2 (SLC7A2) |
F-TGCTCGCGTTCCCAAGA R- GGCCCACAGTTCACCAACAG |
XM_015285435.1 |
| LAT1 | L-type amino acid transporter-1 (SLC7A7) |
F-GATTGCAACGGGTGATGTGA R- CCCCACACCCACTTTTGTTT |
KT876067.1 |
| PepT1 | Peptide transporter-1 (SLC15A1) |
F-TACGCATACTGTCACCATCA R-TCCTGAGAACGGACTGTAAT |
AY029615.1 |
| PepT2 | Peptide transporter-2 (SLC15A2) |
F-TGACTGGGCATCGGAACAA R-ACCCGTGTCACCATTTTAACCT |
NM_001319028.1 |
| GAPDH | Glyceraldahyde -3-phosphate dehydrogenase |
F-GGTGGTGCTAAGCGTGTTA R-CCCTCCACAATGCCAA |
NM205518 |
aThe genes analyzed in the tissues are listed as follow: PGA5 and, PGC in proventriculus; AMY2A, CCK1R, CCK, CELA1, PNLIP, in pancreas; and, GLUT1, GLUT2, CAT1, CAT2, LAT1, PepT1, PepT2 in duodenum