Table 1. Summary of quality of RNA-Seq original sequencing data.

Raw reads column lists each read in the FASTQ format file by four lines and a unit, and counts the number of sequences in each file. Clean reads column lists the filtered sequencing data of raw reads, and the subsequent bioinformation analysis is based on clean reads. Clean bases column lists the number of clean reads multiply the length, and G represents the data size of clean reads. Q20 column listed the percentages of bases with phred quality score greater than 20. Q30 column listed the percentages of bases with phred quality score greater than 20. GC content column lists the total number of bases G and C as a percentage of the total number of bases. Total mapped column lists sequence count statistics that can be mapped to a reference sequence.

Sample name	Raw reads	Clean reads	Cleanbases	Q20(%)	Q30(%)	GC content(%)	Total mapped(%)
larva_1	45,921,768	45,216,478	6.78G	97.84	93.51	39.10	89.14%
larva_2	45,488,706	44,719,458	6.71G	98.15	94.41	39.17	87.93%
larva_3	46,968,766	43,815,018	6.57G	98.20	94.52	39.61	87.88%
pupa_1	51,547,360	50,918,894	7.64G	97.82	93.42	40.09	89.11%
pupa_2	49,213,402	48,731,238	7.31G	97.85	93.58	40.66	86.42%
pupa_3	47,589,126	45,987,494	6.9G	97.88	93.68	41.13	87.12%
female_1	65,486,770	64,749,568	9.71G	97.47	92.46	32.48	91.53%
female_2	54,512,618	51,662,048	7.75G	97.20	92.14	34.17	87.69%
female_3	49,813,910	48,435,084	7.27G	97.31	92.31	34.11	88.84%
male_1	62,939,496	60,971,604	9.15G	97.51	92.88	38.99	84.75%
male_2	69,968,690	69,071,824	10.36G	97.53	92.94	38.33	85.44%
male_3	52,728,692	51,704,548	7.76G	97.66	93.00	34.71	90.75%