Table 3. Purification profile of amylase produced from different Bacillus sp. isolates.

Isolatesa Purification stepb		Bacillus megaterium	Bacillus subtilis	Bacillus cereus	Bacillus thuringiesis	Bacillus lentus	Bacillus alvei
Crude	TP(mg/ml)	0.66	0.96	0.81	0.54	0.99	0.86
	TA(U)	6630	5000	3932	7890	2780	10040
	EA(U/ml)	33.15	25.0	19.66	39.45	13.9	50.2
Ammonium sulfate	TP(mg/ml)	0.58	0.88	0.75	0.39	0.79	0.77
	TA(U)	701.0	640	461.0	820	308	1029
	EA(U/ml)	35.06	32.0	23.08	41.0	15.4	51.47
Dialysis	TP(mg/ml)	0.50	0.82	0.71	0.37	0.60	0.70
	TA(U)	718	700	502	805	365	591
	EA(U/ml)	35.9	35.0	25.12	40.26	18.26	29.56
Sephadex G-200	TP(mg/ml)	0.45	0.76	0.64	0.29	0.48	0.59
	TA(U)	800	760	559	886	456.9	960
	EA(U/ml)	80.03	76.0	55.9	88.64	45.69	96.02

Notes.

^aIsolated Bacillus sp. selected for amylase purification according to SHR.

^bDifferent purification steps of amylase purification.

TP

total protein

TA

total activity

EA

enzyme activity