. Author manuscript; available in PMC: 2020 Nov 5.

Published in final edited form as: Subst Use Misuse. 2019 Jan 20;54(4):670–680. doi: 10.1080/10826084.2018.1531430

Table 1.

List of candidate genes and relative variants analysed, DNA sequence variations and functional consequences. Forward (FW) and reverse (RV) primers used in the PCR reactions, restriction enzymes and references are reported for each variants.

Gene, SNP/VNTR	DNA variation	Functional Consequence	Primer, FW and RV (5’-3’)	Restriction enzyme	Reference
CNR1, rs1049353	A/G (REV)	synonymous codon: Thr ⇒ Thr	FW- GAAAGCTGCATCAAGAGCCC RV- TTTTCCTGTGCTGCCAGGG	MspI	Gadzicki et al., 1999
FAAH, rs324420	A/C (FWD)	missense: Pro ⇒ Thr	FW-ATGTTGCTGGTTACCCCTCTCC RV- TCACAGGGACGCCATAGAGCTG	EcoO109I	Morita et al., 2005
COMT, rs4680	A/G (FWD)	missense, upstream variant 2KB: Val ⇒ Met	FW-TCGTGGACGCCGTGATTCAGG RV- AGGTCTGACAACGGGTCAGGC	NlaIII	Hong et al., 2003
DRD2, rs6277	C/T (REV)^*	synonymous codon: Pro ⇒ Pro	FW-a) ACCACGGTCTCCACAGCACTCT b) ACCATGGTCTCCACAGCACTCT’ RV- ATGGCGAGCATCTGAGTGGCT	Taq^aI / BslI	Hirvonen et al., 2009
ANKK1, rs1800497	C/T (REV)	missense: Glu⇒ Lys	FW-CCGTCGACGGCTGGCCAAGTTGTCTA RV- CCGTCGACCCTTCCTGAGTGTCATCA	Taq^aI	Grandy et al., 1993
DAT1, VNTR 3’UTR	40bp, 3-11 repeats	3’UTR	FW-TGTGGTGTAGGGAACGGCCG AG RV- CTTCCTGGAGGTCACGGCTCAAGG	/	Santtila et al., 2010
DRD4, VNTR exon 3	48bp, 2-11 repeats	exon 3	FW-AGGTGGCACGTCGCGCCAAGCTGCA RV- TCTGCGGTGGAGTCTGGGGTGGGAG	/	Mitsuyasu et al., 2001

^(*)

rs6277 C957T SNP, has been studied in association with the mutation G1101A