Figure 1.

BUB1B is overexpressed in HCC. A, TCGA data analysis of the expression of BUB1B in HCC and normal tissues. B, Expression of BUB1B in HCC and normal tissues analyzed using the GEPIA database. C, Correlation between BUB1B expression and HCC stage was analyzed using the GEPIA database. D, Quantitative RT‐PCR analysis of BUB1B expression in HCC tissues and corresponding normal tissues. E, Western blot analysis of the levels of BUB1B in eight random pairs of HCC tissues (T) and corresponding adjacent normal tissues (N). F, Expression of BUB1B in tumor and normal tissues analyzed by IHC. G,H, Quantitative RT‐PCR and western blot analysis of relative mRNA and protein levels, respectively, of BUB1B in L02 cells and six human HCC cell lines. I,J, Kaplan‐Meier analysis of the relationship of BUB1B with overall survival (i) or recurrence‐free survival (j) in patients with HCC using the GEPIA database. Data represent means ± SD of at least three independent experiments. *P < .05, **P < .01, and ***P < .001