Figure 3.

Overexpression of BUB1B inhibited apoptosis and reduced G0/G1 cell cycle arrest in HCC cells. A, Apoptosis of HCC cell lines with BUB1B knockdown or overexpression analyzed by flow cytometry. B, Western blot analysis of apoptosis‐related proteins in HCC cell lines with BUB1B knockdown or overexpression. C, Cell cycle in HCC cell lines with BUB1B knockdown or overexpression analyzed by flow cytometry. D, Western blot analysis of the levels of checkpoint proteins in the G1/S phase. Data represent means ± SD of at least three independent experiments. *P < .05, **P < .01, and ***P < .001