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. 2020 Sep 12;9(21):8122–8132. doi: 10.1002/cam4.3322

FIGURE 2.

FIGURE 2

LINC00857 inactivates the Hippo signaling pathway and YAP1 acts as an oncogene in ovarian cancer. A, The expression of YAP1 was detected through quantitative real‐time polymerase chain reaction (qRT‐PCR) in SKOV3 and A2780 cells. B, The qRT‐PCR assay was conducted to test the overexpression efficiency of LINC00857 in SKOV3 and A2780 cells via qRT‐PCR. C, The expressions of YAP1, MST1, and LATS1 in the condition of LINC00857 depletion and LINC00857 overexpression were detected respectively through qRT‐PCR analysis. D, The knockdown efficiency of YAP1 in SKOV3 and A2780 cells was tested by qRT‐PCR. E, Cell proliferation was tested by EdU assays when YAP1 was silenced. F and G, Transwell assays were utilized to measure cell migration and invasion after inhibiting YAP1. H and I, TUNEL and flow cytometry assays were implemented to evaluate cell apoptosis when YAP1 was knocked down. J‐L, The F‐FDG uptake, lactate release, and ATP production level were detected in SKOV3 and A2780 cells after silencing YAP1. *P < 0.05, **P < 0.01