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. 2020 Sep 12;9(21):8122–8132. doi: 10.1002/cam4.3322

FIGURE 3.

FIGURE 3

LINC00857 regulates YAP1 by competitively binding to miR‐486‐5p in ovarian cancer. A and B, The distribution of LINC00857 was tested through FISH and subcellular fractionation assays. C, RIP experiment was adopted to detect the enrichment of LINC00857 in Ago2. D, The possible miRNAs were screened out through miRDB. E, The expressions of four miRNAs (miR‐4709‐3p, miR‐486‐5p, miR‐450b‐5p, and miR‐4516) in ovarian cancer cells were detected. F, The overexpression efficiency of miR‐486‐5p in SKOV3 and A2780 cells was tested through quantitative real‐time polymerase chain reaction (qRT‐PCR). G, The expression of YAP1 was tested through qRT‐PCR after overexpressing miR‐486‐5p. H, RIP assay detected the binding situation of YAP1 and miR‐486‐5p. I, RNA pull‐down assay was utilized to prove the combination of LINC00857, YAP1, and miR‐486‐5p. J, The binding sites between LINC00857 and miR‐486‐5p as well as the binding sites between YAP1 and miR‐486‐5p. K, Luciferase reporter assays were utilized to prove the interaction of LINC00857, YAP1, and miR‐486‐5p. L, The knockdown efficiency of miR‐486‐5p was tested through qRT‐PCR. M, The expression of YAP1 was tested when LINC00857 and miR‐486‐5p were inhibited. **P < 0.01