Table 3.
Individual Characteristics and Within-Individual env Diversity Analysis in Seroconverters
| Number of Viral Variants | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Case | Study Site | Estimated Duration of Human Immunodeficiency Virus Infection, da | Age at Enrollment, y | Sex | CD4 Cell Count,b cells/mm3 | Viral Load,c Log10 copies/mL | Maximum (Median) env Genetic Distanced | Model Basede | Phylogeny Basedf |
| P1 | Indonesia | 7 | 22 | F | 527 | 3.7 | 1.1 (0.56) | 1 | 1 |
| P2 | Indonesia | 81 | 36 | M | 726 | 5.2 | 2.6 (0.5) | >1 | 3 |
| P3 | Indonesia | 50 | 29 | M | 338 | 6.7 | 0.57 (0.57) | 1 | 1 |
| P4 | Ukraine | 61 | 31 | F | 486 | 3.7 | 2.4 (0.8) | >1 | 8 |
| P5 | Vietnam | 19 | 22 | M | 522 | 5.2 | 1.1 (0.57) | 1 | 1 |
| P6 | Vietnam | 117 | 27 | M | 223 | 5.6 | 5.3 (2.3) | >1 | 7 |
| P7 | Vietnam | 7 | 32 | M | 501 | 5.1 | 1.7 (0.57) | 1 | 1 |
The table shows demographic characteristics, clinical characteristics, behavioral characteristics, within-individual env genetic diversity, and the number of viral variants detected for 7 partners who acquired human immunodeficiency virus (HIV) infection during the HIV Prevention Trials Network 074 study (seroconverters).
Abbreviation: F, female; M, male; P, partner.
aSamples used for phylogenetic analysis were collected at the first and second HIV-positive visits. The duration of HIV infection was calculated as mid-time point in days between last HIV-negative visit and the first HIV-positive visit, except in 2 cases. Cases P1 and P7 had acute infection at the first HIV-positive visit; in those cases, the estimated duration of infection was classified as 7 days prior to the acute infection visit.
bCD4 cell count testing was performed a median of 5 days after the first HIV-positive visit (range, 2–67 days).
cViral load testing was performed at the first HIV-positive visit, except in 2 cases: case P1 had viral load testing performed 7 days after the first HIV-positive visit; case P7 had viral load testing performed 63 days after the first HIV-positive visit.
dThe env pairwise genetic distances were calculated using the dist.dna function in R “ape” package.
eModel by Keele et al 2008 [28].
fThe number of phylogenetically distinct HIV strains (viral lineages) was determined by visual inspection of Highlighter plots and neighbor-joining phylogenetic trees.