Figure 2. Lower basal and maximal cellular respiration in fresh blood cells obtained from MDS patients as compared to healthy controls.

A. OCR measurements of peripheral blood cells obtained from an MDS patient (Red) in comparison to his matched healthy control (blue) under the same conditions. Cells were plated on a Cell-Tak™ coated 24 well XF V7 cell culture microplate at 0.5×10⁶ cells per well in 50 μL of XF assay medium. Each dot represents the average of 3 repeats. B. Results shown are an average of 20 MDS patients and 20 healthy controls comparing the basal respiration (two left columns) and maximal respiration (two right columns) of the healthy controls (Blue) and MDS patients (Red). The error bars represent the standard deviation. Cellular respiration was assessed in fresh blood cells (two hours after collection from the patients at the hospital) using the Seahorse XF analyzer