FIG 1.

In the absence of Spo0A, C. difficile produces elevated levels of SinR. (A) qRT-PCR results of sin locus transcripts in C. difficile strains collected at the 10-h time point. The representative results from three independent experiments are shown. The asterisks (***) indicate statistical difference at P < 0.005. (B) Western blot analysis of parent strains (R20291, JIR8094, and UK01) and their respective spo0A mutants and complemented strains using SinR- and Spo0A-specific antibodies, demonstrating upregulated SinR in the absence of Spo0A. The sinR mutant served as a negative control. Glutamate dehydrogenase (GDH) detection using anti-GDH antibodies was used as loading control.