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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Dev Biol. 2020 Aug 19;467(1-2):30–38. doi: 10.1016/j.ydbio.2020.07.013

Figure 3. Expression of important diaphragm genes is maintained in E12.5 PPFs during culture.

Figure 3.

Expression of Gata4 (A), Gata62 (B), Zfpm (C), Tbx5 (D), and Nr2f2 (E) does not statistically differ in E12.5 PPFs when cultured 5 or 10 d. PDGFRa is reduced after 5 d in culture, but is not significantly reduced after 10 d in culture (F). NIH 3T3 fibroblasts have lower expression of Zfpm2 (C) and undetectable levels of Tbx5 (D). For each experiment, 3 pairs of E12.5 PPFs were pooled or 3 wells of 3T3s were pooled and then 2-5 pools were used for qPCR. Gene expression determined by qRT-PCR, normalized against 18S gene expression, and then normalized to “at harvest” value, which was set to 1. Significance tested with one-way ANOVA. Holm–Sidak post hoc tests are indicated by * p<0.05, **** p<0.0001. Error bars represent standard error of the mean (SEM).