Fig. 5.

Expression of virulence factors and pathogenicity. (a) The hcn operon in CLJ1 and insertion of CLJ-ISL3 into the hcnB gene in CLJ3. (b) Relative expression of hcnB measured by RT-qPCR and HCN production in the strains indicated. Statistical significance was determined using a t-test. ***P<0.001. HCN production was detected by placing a paper impregnated with a reaction mixture containing Cu²⁺ ions above agar plates seeded with bacteria. A white-to-blue colour transition indicates the presence of HCN in the gas phase. (c) RT-qPCR analysis of the relative expression of ccoG2, ccoN4, lptF and CLJ1_1187 in CLJ1 and CLJ3 strains. Bars, sem. Statistical significance was determined using a t-test. **P<0.01 and ***P<0.001. (d) Insertion of CLJ-ISL3 into the imuABC operon. (e) Survival of Galleria following injection of different strains. Twenty larvae were infected with 5–10 bacteria (estimated from c.f.u. counts) and their survival was monitored over the indicated period. PBS was injected as a vehicle-control. ***P<0.001.