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. 2020 Nov 5;15(11):e0241818. doi: 10.1371/journal.pone.0241818

Fig 6. Caffeine reversed light anesthesia equally well in both male and female rats.

Fig 6

Two small groups, n = 4, of male and female rats were anesthetized using the protocol described in Fig 1. Both groups were anesthetized twice, once with a saline infusion and once with caffeine (37.5 mg/ kg). The Δ in isoflurane between caffeine and saline was ~0.4% isoflurane. Using a 2-way ANOVA with multiple comparisons and Tukey’s multiple comparison test, no significant differences between male and female rats were found. Female saline vs. female caffeine, p = 0.0081. Male saline vs. male caffeine, p = 0.0097. Female saline vs male saline, p = 0.1. Female caffeine vs male caffeine, p = 0.15. Isoflurane RORR concentrations in saline treated males: 0.5, 0.55, 0.6, 0.55. Isoflurane RORR concentrations in caffeine treated males: 0.75, 1.05, 1, 0.95. Δ for males: 0.25, 0.5, 0.4, 0.4. Isoflurane RORR concentrations in saline treated females: 0.25, 0.4, 0.35, 0.55. Isoflurane RORR concentrations in caffeine treated females: 0.65, 0.75, 0.8, 1.0. Δ for females: 0.4, 0.35, 0.45, 0.45.