Fig 4. Aptamer binding to native and differentially heat-treated rituximab using ELASA.

Shown are the means and standard deviations of six replicates per sample and aptamer. One replicate value had to be omitted from sample 80°C 1 min for aptamer C10 and C57 respectively, because they were disproportionately low and likely inconclusive for the measurement. A non-parametric test was performed, comparing all samples to each other, with corrections for multiple comparisons according to Dunn (*, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001).