Table 1. Aptamer sequences obtained after SELEX using heat-treated rituximab.
| aptamer | sequence | frequency | G-score | G-C content (%) |
|---|---|---|---|---|
| C2 | TAGTATGGACTTGTCTTCTTGTCAGAGTCTGGCAGCACGT | 3/46 | - | 47.5 |
| C4 | GGCACTTGTTTGCTCGAACGATGGTTTGTGCTTTTGTGTT | 2/46 | - | 45 |
| C5 | CGCTCCGTTTACTCGGTCTGCTATTGGCTTGCCTTTGTTT | 6/46 | - | 50 |
| C7 | GGCCATTGTGGACTTCTTTGGGTAATTCAGGGGCTCGATT | 5/46 | 30 | 50 |
| C10 | ACTTCGGCTAGTTAGGGGGTAGTTTAGATCGTCTCTACAT | 2/46 | - | 45 |
| C57 | GGCGTATGCAGTTTTGTAGGGTTATCAAGGCTTGACGATT | 3/46 | - | 45 |
Highlighted in bold and underlined are guanine bases (G) predicted to be involved in the formation of G-quadruplex structures. The G-score value represents the probability of quadruplex formation by G-rich sequences (maximum value obtained with default settings is 105).