Fig 5. Electropherogram alignment enables the comparison of peaks and potential identification of biomarkers.

Shown is a time-expanded view (along the X-axis) of the averaged electropherograms (EPG) derived in the three aqueous humor sample categories, i.e., from the non-transplanted controls and from transplanted mice that either rejected or tolerated their islet allografts. Comprehensive one-way analysis of variance (ANOVA) of height of the individual peaks (each corresponding to a putative analyte) identified 23 significant differences in peaks that clearly discriminated between the sample classes. Six of most significant peaks are labeled in the figure. Notably, these peaks correspond to putative analytes which can potentially be used as biomarkers of islet allograft immune-mediated rejection versus tolerance.