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. 2020 Nov 5;15(11):e0241978. doi: 10.1371/journal.pone.0241978

Fig 4. Confocal laser scanning microscopy of LIVE/DEAD-stained E. coli biofilms.

Fig 4

Pre-formed biofilms (3 d-old) of the MDR ESBL-producing strain Ec AM7 were incubated for 1 h with (A) M9 medium (untreated), (B) 0.25% ethanol (vehicle), or (C) 1% (v/v) 1,8-cineole and were further incubated with the Live/Dead viability stain to show live (green) or dead (red/yellow) bacterial cells. Scale bars: 20 μm. (D) COMSTAT analysis of biomass. For each condition, the % of live and dead bacteria was calculated.