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. 2020 Oct 26;16(10):e1009102. doi: 10.1371/journal.pgen.1009102

Fig 1. Alternative transcription analysis of unc-68 through fluorescent reporter knock-ins.

Fig 1

(A) Schematic of the C. elegans unc-68 locus (exons in black, introns and promoters in white), highlighting alternative transcription start sites and location of fluorescent protein sequence knock-ins made by CRISPR/Cas9-mediated genome editing. mNG: mNeonGreen. (B, C, D) Representative fluorescence and DIC images taken in the head (B, D) and midbody (C) of wild type, [mScarlet::unc-68ex1.1] (B, C), and [mNeonGreen::unc-68ex1.2] (D) transgenic animals. BWM: Body wall muscle; Vu: Vulval muscle; *, autofluorescence background in the intestine. Scale bar: 50 μm.