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. 2020 Oct 26;16(10):e1009102. doi: 10.1371/journal.pgen.1009102

Fig 4. Expression of unc-68 alternative exon 13 in specific tissues.

Fig 4

The expression of unc-68 exon 13 was analyzed using alternative splicing reporter minigenes. (A, D) Schematic of the monochromic (A) and bichromic (D) unc-68 exon 13 alternative splicing reporter minigenes and of the expression system. The Q-system (including unc-68p2::QF and QUAS::’reporter’ transgenes) was used to drive the transcription of each minigene into pharyngeal muscle and neurons. For each of them, exon 13 inclusion yields transcripts with an in frame mScarlet sequence, and its exclusion yields transcripts with an in frame CeBFP sequence. (B, C, E) Representative fluorescence images of transgenic animals expressing the monochromic (B, C) and bichromic (E) unc-68 exon 13 alternative splicing reporters. Arrow heads: unidentified neuronal cells. *, autofluorescence background in the intestine. Scale bar: 50 μm.