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. 2020 Oct 26;16(10):e1009007. doi: 10.1371/journal.ppat.1009007

Fig 6. PfMyoA drives invasion pore closure, while PfMyoA and PfMyoB both help initiation of internalisation.

Fig 6

A RAP treated PfMyoA-K764E parasites showed a significantly shifted distribution of event types, with more Type B and Type C events. This is consistent with these parasites having insufficient motor function to overcome a third barrier, at completion of internalisation. Significance assessed by chi-square test. PfMyoB-cKO parasites showed a significant increase in Type C failures, consistent with an impairment at initiation of internalisation. Significance assessed by Fisher’s exact test, comparing successful invasion to pooled invasion failures. B PfMyoA-K764E parasites show a slight weakening of deformation, though not significant. There is no difference between the deformation scores in PfMyoB-cKO parasites after RAP treatment. Significance assessed by chi-square test. C For PfMyoA-K764E parasites undergoing Type A events (black bars and data points) RAP treatment induces a longer pause pre-internalisation. Only in Type B events (cyan bars) after RAP treatment is internalisation significantly slower and the pause post-internalisation shorter. Bars show median and interquartile range, or median only for Type B events. Significance assessed between Type A DMSO and RAP treatments by Mann-Whitney test, shown when p<0.5. D The duration of pre-internalisation pause is significantly increased in PfMyoB-cKO parasites, suggesting that PfMyoB plays a role in initiation of internalisation. The post-internalisation pause is reduced by a similar amount. Bars show median and interquartile range. Significance assessed by Mann-Whitney test, shown when p<0.5. E Example of a RAP-treated PfMyoA-K764E merozoite, undergoing a Type B failure, showing apparent completion of internalisation before subsequent ejection (S3 Video), and a PfMyoB-cKO merozoite undergoing successful invasion (S4 Video). Time indicated in seconds, scale bar 2 μm. F Schematic based on Fig 4A showing that PfMyoA-K764E merozoites can proceed to internalisation, but are frequently ejected, while PfMyoB-cKO merozoites invade successfully, but with delayed initiation of internalisation.