Figure 5.

CircMAT2B knockdown decreased OGD-induced H9c2 cells inflammatory injury via up-regulating miR-133. (a) Level of miR-133 was examined via qRT-PCR after transfection with miR-133 inhibitor. (b) Cell viability was examined through CCK-8. (c) Apoptosis was examined through flow cytometry. (d,e) Levels of apoptosis-related factors were examined through western blot analysis. (f) ROS generation was examined via flow cytometry using DCFH-DA. Expression of IL-1β, IL-6 and TNF-α was examined via western blot (g) and ELISA (h). * P < 0.05, ** P < 0.01 and *** P < 0.001 contrasted with indicated set