Figure 4.

Impact of melatonin on expression of AMPK and apoptotic proteins. Cells in each group were treated with 4 mmol/L of Ox for 12 h (except the NC group). Western blot and corresponding histograms of protein expression levels of caspase-12, cleaved caspase-3, cleaved caspase-9, p-AMPK, and AMPK relative to GAPDH. Data are presented as the mean ± SEM from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 versus the NC group; #P < 0.05, ##P < 0.01, ###P < 0.001, ####P < 0.0001 versus the Ox group; ns: not significant