Figure 6.

WT1 depletion reduces SKOV3 cells metastasis in vivo. (a) Western blotting was performed to confirm the knockdown efficiency of WT1 in established tumors. (b) Tumor growth curves were summarized in the line chart. (c) Image of metastatic liver nodules spreading throughout the live tissues in mice injected with shRNA control SKOV3 cells. WT1 knockdown clearly abolished the metastasis. White arrows indicated tumor foci. Bar = 1 mm. (d) Hematoxylin and eosin (H&E) staining was used to visualize the metastatic nodules in tumors from mice injected with shRNA control SKOV3 cells. The arrow indicated tumor foci. Magnification x 20. Bar = 50 μm. E, Western blot was performed to detect changes in the protein expression of EMT-associated genes proteins and ERK1/2 activation in the xenograft mouse primary tumors from the mice inoculated with WT1 KD cells compared to the controls. The lower panel of figure A or the panel on the right of figure E showed the quantification of the results. ** P < 0.01