Figure 2.

MiRNA-324-5p has an oncogenic effect in GC

(A) MiRNA-324-5p promoted cell proliferation. Cell proliferation was measured using CCK8 assay in BGC-823 cells with NSC-inh (nonspecitic control) or miRNA-324-5p inhibitors transfection. (B)The expression level of miRNA-324-5p was markedly decreased in BGC-823 cells by chemically synthesized miRNA-324-5p inhibitors. (C) MiRNA-324-5p promoted cell migration. Cell migration was measured by transwell assay in BGC-823 cells with NSC-inh or miRNA-324-5p inhibitors transfection. Cells were counted from five randomly chosen fields. (D) MiRNA-324-5p inhibitors resulted in decreased tumor volumes in vivo. Tumor growth curves are shown with volume measured twice a week after injection of either NSC-inh or cholesterol-conjugated 324-inh. Each experiment was repeated three times. Student t test was performed.*p < .05.