Figure 5.

MiRNA-324-5p activates Wnt/β-catenin signaling pathway and EMT via SUFU. (a) Topflash luciferase activity was decreased by miRNA-324-5p inhibitors and SUFU siRNAs partially reversed this inhibitory effect. BGC-823 cells were transfected with miRNA-324-inh or co-transfected with miRNA-324-inh and SUFU siRNAs. (b) Interference of SUFU relieved the cytoplasmic retention of β-catenin induced by miRNA-324-5p blockage. (c) β-catenin translocation to nuclei induced by miRNA-324-5p inhibition was reversed by the coupled SUFU knockdown. Confocal immunofluorescence was performed to locate the β-catenin cytoplasmic or nuclear localization 48 h after transfection. (d) Suppression of SUFU alleviated the EMT induced by miRNA-324-5p inhibition. Each experiment was repeated three times