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COX inhibition increased CysLT release in WT mice and augmented IL-33 release in WT and 5-LO KO mice. WT BALB/c and 5-LO KO mice were treated with Indo or Veh and challenged intranasally with one dose of HIA or Alt. Mouse BALF was harvested 1h after the challenge for CysLT and IL-33 ELISA. (A) The levels of CysLTs. (B) IL-33 levels in WT mice. (C) IL-33 levels in 5-LO KO mice. Combined data of 2 independent experiments were shown. * p<0.05, n=3–16 mice.
