Table 3.
Universal primers for V3 – V4 region of the bacterial 16S rRNA gene
| Sample information | Forward primer (341 F) 5’-> 3’ |
Reverse primer (805 R) 5’-> 3’ |
Forward index |
Reverse index |
|---|---|---|---|---|
| Normal control 1 | CCTACGGGNGGCWGCAG | GACTACHVGGGTATCTAATCC | CTCTCTAT | TCGCCTTA |
| Normal control 2 | CCTACGGGNGGCWGCAG | GACTACHVGGGTATCTAATCC | CTCTCTAT | CTAGTACG |
| Normal control 3 | CCTACGGGNGGCWGCAG | GACTACHVGGGTATCTAATCC | CTCTCTAT | TTCTGCCT |
| Western diet 1 | CCTACGGGNGGCWGCAG | GACTACHVGGGTATCTAATCC | CTCTCTAT | GCTCAGGA |
| Western diet 2 | CCTACGGGNGGCWGCAG | GACTACHVGGGTATCTAATCC | CTCTCTAT | AGGAGTCC |
| Western diet 3 | CCTACGGGNGGCWGCAG | GACTACHVGGGTATCTAATCC | CTCTCTAT | CATGCCTA |
| L. lactis 1 | CCTACGGGNGGCWGCAG | GACTACHVGGGTATCTAATCC | CTCTCTAT | TCCTCTAC |
| L. lactis 2 | CCTACGGGNGGCWGCAG | GACTACHVGGGTATCTAATCC | CTCTCTAT | TCATGAGC |
| L. lactis 3 | CCTACGGGNGGCWGCAG | GACTACHVGGGTATCTAATCC | CTCTCTAT | CCTGAGAT |