Figure 7. Characteristics of eGenes are consistent with less constraint on eGene expression.

(A) eGenes are more differentially expressed between species than non-eGenes, with eGenes detected in both species being even more differentially expressed. The distribution of the inter-species differential expression effect size is plotted for each eGene group as an ECDF. (B) eGenes are more diverged at amino acid level than non-eGenes. (C) Human-specific eGenes and shared eGenes are more divergent than expected under neutrality. The analogous test for chimpanzee-specific eGenes displayed a shift that was not statistically significant, although our classification of eGenes in chimpanzee may be underpowered. p-Values provided for one-sided Mann-Whitney U-tests with the noted alternative hypothesis.

Figure 7—figure supplement 1. Gene categories of human-chimpanzee shared and chimpanzee-specific eGenes.

(A) Gene ontology categories enriched amongst the 21 eGenes identified in both species (foreground), compared to the 734 eGenes identified in only chimpanzee or only human (universe/background). Among the significant categories (Adjusted p-value<0.05), only the top five most enriched gene categories for each ontology set are shown for space. (B) Gene ontology categories enriched amongst the 148 eGenes identified only in chimpanzee, compared all 6797 eGenes identified in either species using the full GTEx dataset (universe/background). All significant categories are shown. Full GO enrichment results for (A) available as Figure 7—figure supplement 1—source data 1 and for (B) as Figure 7—figure supplement 1—source data 2.