Key resources table.

Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
Antibody	Anti-GFP antibody (chicken polyclonal)	Aves Labs	GFP1010 RRID:AB_2307313	(1:1000)
Antibody	Alexa Fluor 488 goat anti-chicken IgG (goat polyclonal)	Thermo Fisher Scientific	A32931 RRID:AB_2762843	(1:500)
Antibody	Rabbit anti-PV (rabbit polyclonal)	Swant	PV-27 RRID:AB_2631173	(1:1000)
Chemical compound, drug	BDA (10 KDa)	Thermo Fisher Scientific	D1956	3% solution
Chemical compound, drug	Hoechst 33342	Thermo Fisher Scientific	H3570	1:1000 solution
Software, algorithm	ImageJ	NIH
Software, algorithm	Imaris	Oxford Instruments		Version 9.3.2
Software, algorithm	Prism 8	Graphpad		Version 8.0
Other	EnvA-RVdG-mCherry	Gift; Dr. Ian Wickersham, MIT Chatterjee et al., 2018		Rabies virus expressing mCherry
Other	AAV1-TREtight-B19G	Gift; Dr. Ian Wickersham, MIT Chatterjee et al., 2018		AAV expressing B19 variant of G protein
Other	AAV1-syn-FLEX-TA-TVA-GFP	Gift; Dr. Ian Wickersham, MIT Chatterjee et al., 2018		AAV expressing cre dependent TVA, tTA, and GFP
Other	AAV2/8-CAG-pre-mGRASP-mCerulean	Gift; Dr. Michael Baratta, Univ. of Colorado	N.A.	AAV expressing pre-mGRASP and mCerulean
Other	AAV2/8.CAG.Jx-rev.post-mGRASP-2A-dTomato	Gift; Dr. Michael Baratta, Univ. of Colorado	N.A.	AAV expressing cre dependent post-mGRASP and tdTomato
Other	AAVrg-hSyn-Cre-WPRE-hGH (retrograde)	Addgene Vector Core	Lot#: 105553-AAVrg	AAV expressing cre recombinase
Other	AAV1-hSyn-Cre-WPRE-hGH	Addgene Vector Core	Lot#: 105553-AAV1	AAV expressing cre recombinase
Other	AAV1-pCAG-FLEX-tdTomato-WPRE	Addgene Vector Core	Lot# 51503	AAV expressing cre dependent tdTomato
Other	AAV1-CamKIIa-SSFO-GFP	UNC vector core	N.A.	AAV expressing SSFO and GFP under CamKII promoter
Other	pENN-AAV-CamKII-0.4.-Cre-SV40	Addgene Vector Core	Lot#: 105540	AAV expressing cre under CamKII promoter