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. Author manuscript; available in PMC: 2021 Apr 24.
Published in final edited form as: J Nat Prod. 2020 Mar 20;83(4):937–954. doi: 10.1021/acs.jnatprod.9b00773

Table 1.

Inhibition of Tubulin Polymerization and Colchicine Binding

compound inhibition of tubulin polymerizationa IC50 inhibition of colchicine bindingb % inhibition ± SD
(μM)±SD 1 μM 5 μM
29 (CA1)c 1.9 NDd 99.6±0.7
42 (CA4) 0.64±0.01 84±2 97±0.7
20 0.84±0.1 50±5 84±1
21 0.82±0.04 72±4 94± 0.7
22 >20
23 >20
24 12±1
25 >20
26 9.5±0.9
35 1.7±0.2 25±3
36 >20
37 1.7±0.01 53±3 92±0.5
38 0.84±0.1 34±3 90±0.7
39 4.3±0.4 58±4
40 6.2±0.3 72±3
41 1.3±0.08 43±4
43 >20 26±1
44 >20 15±5
45 >20 33±3
a

Average of n ≥ 2 independent determinations.

b

Only compounds showing significant activity as inhibitors of tubulin polymerization (defined as having IC50 values below 5 μM) were evaluated for inhibitory effects on colchicine binding.

c

Data from refs. 44, 26, and 48.

d

ND= not determined.